Detection of expression level of protein P16 in lung neoplasms biopsy samples by flow cytometry
- VernacularTitle:流式细胞术检测肺癌患者P16蛋白表达水平的应用价值
- Author:
Zongli SHEN
;
Yueqing ZHU
;
Yiping ZHUANG
;
Xiaoliu WU
;
Yaping WANG
- Publication Type:Journal Article
- Keywords:
Flow cytometry;
Lung neoplasms;
Protein P16
- From:
Chinese Journal of Laboratory Medicine
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To improve the sensitivity of detection of protein P16 by FCM.Methods Protein P16 of blood cells and tumor cells in biopsy samples were labeled simultaneously with anti-CD45 and anti-P16 fluorescent antibody.The rates of CD45+P16+ cells and CD45-P16+ cells were calculated by FCM.Results The low expression rates of protein P16 in normal blood cells and in tumor cells in biopsy samples were 15.39%(6/39) and 69.23%(27/39),respectively, which had a significant differences (P
