An entirely automatic system of nucleic acid amplification testing in blood screening and follow up study of positive donors
- VernacularTitle:全自动血液核酸筛查及阳性献血员追踪的研究
- Author:
Xianlin YE
;
Lianghua WANG
;
Yiyan ZHOU
;
Lixin YANG
;
Guifang SHANG
;
Weigang ZHU
- Publication Type:Journal Article
- Keywords:
Nucleic acid amplification techniques;
Automation;
Blood donors
- From:
Chinese Journal of Laboratory Medicine
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the entirely automatic method of nucleic acid amplification testing (NAT) for blood screening and to study the feasibility of NAT.Methods Using entirely automated extraction method to extract nucleic acid , amplified and detected by Roche COBAS AMPLICOR system,evaluating the sensitivity and efficacy.Results The 95% limits of HBV DNA, HCV RNA/HIV-1 RNA tests by automation system were 38.9,16.4IU/ml and 20.4 copies/ml,95% Confidence Intervals were [21,323], [10.5,342] and [12,300] respectively.8 of 16 512 donations were PCR for HBV DNA positive,the DNA positive rate was 0.048%.7/8 donations were Anti-HBc positive,The last one was also converted positive.No positive HCV RNA and HIV RNA was detected. 3/6 following up samples seroconverted.Conclusions The entirely automatic system can be applied in blood screening.
