Brg1 gene mutation and the protein expression in gastric carcinoma
- VernacularTitle:胃癌组织中Brg1基因突变及蛋白表达的研究
- Author:
Qing LI
- Publication Type:Journal Article
- Keywords:
Gastric adenocarcinoma;
Brg1 protein;
Gene mutation;
Immunohistochemistry
- From:
Journal of Medical Postgraduates
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mutation of Brg1 gene and expression of Brg1 protein in gastric adenocarcinoma,and to study its relation with tumor development.Methods:By means of polymerase chain reaction-single strand conform action polymorphism(PCR-SSCP)37 cases of gastric mucosa were searched for Brg1 gene mutation in exon 4 and 16.By immunohistochemistry the expression of Brg1 protein in 30 cases of gastric adenocarcinoma was analyzed for their role in gastric cancer development and prognosis.Results:In 37 cases of gastric adenocarcinoma,no mutation of Brg1 gene was detected.Brg1 protein expression was 76.67% in gastric carcinoma which was higher than that in normal tissue(44.44%.) The expression of Brg1 was related to tumor growth,lymphnode metastasis and tumor differentiation.Conclusion:Genetic abnormalities of Brg1 might be rare and might not play a major role in stomach carcinogeneses.An increased expression of Brg1 protein was associated with the advanced stage of gastric carcinoma,the Brg1 expression can be used as an important indicator for differentiating malignancy and prognosis of gastric carcinoma.
