The Therapentic Effect of Influenza A Virus on Murine S180 Ascites Tumor
- VernacularTitle:甲型流感病毒治疗小鼠S180腹水瘤的研究
- Author:
Guoyu ZENG
;
Yanfei XIN
;
Mingyuan LI
;
Hong LI
;
Liying XIAO
;
Zhonghua JIANG
- Publication Type:Journal Article
- Keywords:
influenza A virus;
S180 ascites sarcoma;
cytokine;
apoptosis
- From:
Chinese Journal of Cancer Biotherapy
1995;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the immunological mechanism of influenza A virus for murine S180 ascites sarcoma. Methods: After inoculation with S180 sarcoma cells, mice were i. p. injected with influenza A virus or vehicle 15 days. The average living time and survival rate of the mice were examined. The levels of IL-2, IL-6 and TNF-? were detected. The sarcoma cell's apoptosis was detected by DNA ladder, flow cytometry (FMC) , fluorescent microscope and e-lectron microscope (EM). Results: The average living time and survival rate of the mice injected with Influenza A virus were significantly longer or higher than that of the controls. The levels of IL-2, IL-6 and TNF-? also had the same differences. The apoptosis cells were detected by EM and fluorescent microscope. Sub-diploid peaks were observed by FCM a-nalysis and DNA ladder was seen after electrophoresis in the ascites cells. Conclusion: Our results demonstrated that the feasibility and potential of delivery of influenza A virus as a general means for the treatment of S180 ascites sarcoma.
