In vitro susceptibility testing of yeasts: comparison of E-test and disk diffusion method
- VernacularTitle:氟康唑纸片扩散法和浓度梯度法检测酵母菌耐药性的研究
- Author:
Xinmao ZHAO
- Publication Type:Journal Article
- Keywords:
Fluconazole;
Microbial sensitivity test;
Yeasts
- From:
Chinese Journal of Laboratory Medicine
2003;0(09):-
- CountryChina
- Language:Chinese
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Abstract:
Objective To study on the correlation between E-test method and disk diffusion method for determining fluconazole.Methods Fluconazole Disk Diffusion and E-test method procedures were used for determining susceptibility of 142 yeasts isolated from the Peking Union Medical college Hospital. The diameter of inhibition zones of test and reference strain were read by BIOMIC System.WHONET-5 software was used for analyzing the results and evaluating the correlation of in-vitro susceptibility tests. The two susceptibility tests were performed using NCCLS Standard.Results A comparison of the cumulative fluconazole MICs reported by two methods showed a high correlation, accounting for 82.4% of all the isolates. Very major error was observed (0.7%),major errors was observed (2.8%) and minor errors (14.7%) were found . The results of candida glabrata have a few difference by two in-vitro susceptibility tests.Conclusions Although Disk diffusion method showed the better agreement with the E-test method for almost yeasts, a few of yeasts isolated need the procedure of NCCLS broth macrodilution for determing MICs.
