Effects of different Helicobacter pylori phenotype on gastric mucosal pathogenesis
- VernacularTitle:不同表型幽门螺杆菌对胃黏膜ki-67表达的影响
- Author:
Faming ZHANG
- Publication Type:Journal Article
- Keywords:
Helicobacter pylori;
Phenotype;
Chronic gastritis;
Gastric cancer;
ki-67
- From:
Journal of Medical Postgraduates
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of different Helicobacter pylori (Hp) phenotype on expression of ki-67 in gastric mucosa. Methods:137 patients (with Hp infection) with chronic gastritis(CG), peptic ulcer or gastric cancer and 34 patients without Hp infection were observed. All patients with gastroduodenal diseases were diagnosed by endoscopy and histology. Western blotting was used to detect phenotypes of Hp Cytotoxin-associated protein (CagA), vacuolating cytotoxin (VacA), Urease and their sub-phenotypes. In Hp associated gastritis, histological grade of chronic inflammation (CI), polymorphonuclear neutrophil activity(PA) and density of Hp (DH) were scored according to the Updated Sydney System. Immunohistochemistry was used to exam the expression of ki-67. Results:①Expression rates of Hp128000CagA, 116000CagA, 95000VacA, 91000VacA and 30000UreA in the group of mild grade of CI were lower than those in the moderate-marked group (P(0.05,)respectively).③In Hp(+) CG, (ki-67LI) in the group with expression of (128000)CagA, 95000VacA or 30000UreA were higher than that in groups without their expression. In Hp(+) gastric cancer, (ki-67LI) in the group with expression of 128000CagA was higher than that in the group without its expression((P=)0.02). Conclusion:Many virulent factors of Hp may cause increased grade of CI and play an important role in the occurrence and development of PA. Hp infection and density of Hp seem to cause increased epithelial cell proliferation in gastric mucosa with chronic gastritis. But they may exert different influences on malignant and benign gastric mucosa, and more virulent factors probably increase epithelial cell proliferation in gastric mucosa with chronic gastritis rather than gastric cancer.
