Screening antarctic bacteria producing EPA and analyzing its composition of fatty acids
- VernacularTitle:产EPA南极细菌的筛选及其脂肪酸成分分析
- Author:
Guodong WANG
- Publication Type:Journal Article
- Keywords:
antarctic bacteria;
gas chromatography/mass spectrometry;
EPA;
unsaturated fatty acid
- From:
Chinese Journal of Marine Drugs
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen antarctic bacteria producing EPA and analyse its composition of fatty acids.Methods Two hundred strains of bacteria were isolated from ice and soil of the Antarctic.The total lipid was extracted by using a modified one-phase Bligh and Dyer extraction,and screened by means of GC using fish oil as standard.In the end,eight strains of bacteria were screened which had high content of EPA or DHA.Among them,one bacterium(NJ136) which had the highest content of EPA was obtained,its total lipid was extracted by means of Soxhlet extractor,and the compositions of fatty acids were analysed by means of GC-MS.Results and conclusion The relative contents of EPA and total unsaturated fatty acids of NJ136 were 6.66% and(51.92%) respectively,the content of total fat,EPA and unsaturated fatty acids of dry bacteria weight were 7.94%,5.3 mg?g~(-1) and 41.2 mg?g~(-1),respectively.
