Establishment and Evaluation of a Rat Model of Peritoneal Bacterial Infection after Liver Transplantation
- VernacularTitle:大鼠肝移植术后腹腔细菌感染模型的建立与评价
- Author:
Long DING
;
Yu YANG
;
Jiahong DONG
- Publication Type:Journal Article
- Keywords:
Liver transplantation;
Immunological sensitization;
Bacterial infection,peritoneal;
Rat
- From:
Acta Laboratorium Animalis Scientia Sinica
2010;18(1):69-73,彩7
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a rat model of peritoneal bacterial infection after liver transplantation.Methods To construct a dark Agouti rat-to-Lewis(DA-to-LEW) rat model of liver transplantation.Peritoneal bacterial infection in the rats was induced by intraperitoneal injection of bacterial suspension.The liver function,blood gas,blood cell count and other indicators of the rat models were detected.Results There was a high mortality rate in rats with bacterial injection at day 5 after liver transplantation,therefore unfavorable for the following study.It waft better to inject the bacteria in an amount of 5×10~5 cfu/mL at day 3 after liver transplantation.The cumulative 7-day survival rate of those rats after infection reached up to 37.5%.The infection became increasingly severe,the general conditions were worsening,the rectal temperature was rising,the WBC count was increased,the pH was decreased,liver dysfunction was progressively increased,and metabolic acidosis occurred in the rats.Liver parenchymal damage was more pronounced than that of bile ductal injuries,and the rats died one after another at about 5 days after infection.Pathological examination of multiple organs showed that the main cause of death of the rats was liver damage,without accompanying lung and kidney damages.Conclusion The results of this study suggest that it is a successful method to establish a rat model of peritoneal bacterial infection after liver transplantation,and this model can be used in related experimental researches.
