Effect of NF-κBp65 on Epithelial-mesenchymal Transition in Esophageal Squmous Cell Carcinoma
10.3969/j.issn.1000-8179.2010.04.003
- VernacularTitle:NF-κBp65对食管鳞癌细胞上皮间质转化的影响
- Author:
Hongyan ZHANG
;
Xiaojun WANG
;
Feng WANG
;
Xinhua WANG
;
Yang SUN
;
Shanshan LI
- Publication Type:Journal Article
- Keywords:
Epithelial-mesenchymal transition;
NF-κBp65;
Esophageal squamous cell carcinoma
- From:
Chinese Journal of Clinical Oncology
2010;37(4):187-189,193
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect of NF-κBp65 on epithelial-mesenchymal transition (EMT) in esophageal squa-mous cell carcinoma cell line EC9706. Methods: Esophageal squamous cell carcinoma cell line EC9706 was transfected by chemically synthesized NF-κBp65-ASODN. RT-PCR, immunocytochemistry and flow cytomertry were used to detect the mRNA and protein expression of NF-κBp65, E-cedherin and Vimentin in EC9706 before and after NF-κBp65-ASODN trans-fection. The morphological alterations of EC9706 cells were observed under inverted microscope and scarification test was used to detect the mobility of EC9706 cells before and after transfection. Results: After NF-κBp65-ASODN transfection, the mRNA (0.14±0.06) and protein (immunocytochemistry: 11.33%±1.40%, flow cytomertry: 11.78%) expressions of NF-κBp65 in EC9706 were significantly lower than those before transfection (mRNA: 0.45 ± 0.05; protein: immunocytochemistry: 17.17%±1.66%, flow cytomertry: 15.76%, P<0.05), the mRNA (0.36±0.08) and protein (immunocytochemistry: 17.58%± 1.86%, flow cytomertry: 14.98%) expressions of E-cedherin in EC9706 cells were significantly higher than those before transfection (mRNA: 0.22±0.06; protein: immunocytochemistry: 14.42%±1.63%, flow cytomertry: 12.22%, P<0.05) and the mRNA (0.75±0.07) and protein (immunocytochemistry: 16.00% ± 1.41 %, flow cytomertry: 12.90%) expressions of Vimentin were significantly lower than those before transfection (mRNA: 0.89±0.09; protein: immunocytochemistry: 19.50±0.89%, flow cytomertry: 17.76%, P<0.05). After NF-κBp65-ASODN transfection, the morphological alterations of EC9706 cells oc-curred and the migration length (0.45±0.08) was significantly shorter than that before transfection (0.81±0.11, P<0.05). Con-clusion: NF-κBp65 may contribute to EMT in esophageal squamous cell carcinoma. NF-κBp65-ASODN transfection can in-hibit EMT in esophageal squamous cell carcinoma, up-regulate E-cadherin expression, down-regulate Vimentin expression and decrease cell mobility.
