Construction of inducible lentiviral expression vector containing MST1 gene and its expression in Huh-7 cell line
- VernacularTitle:构建可诱导表达MST1基因的慢病毒载体及其在肝癌细胞系Huh-7中的表达
- Author:
Bo GU
- Publication Type:Journal Article
- Keywords:
inducible lentiviral vector;
MST1 gene;
doxcycline
- From:
Fudan University Journal of Medical Sciences
2010;37(1):76-79,99
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct inducible lentiviral vector containing MST1 gene, which provides an ideal model for further study of the relationship between liver cancer and MST1 gene. Methods We cloned MST1 into inducible lentiviral vector. Two lentiviral vectors (pLVPT-tTRKRAB-MST1 and pLV-tTRKRAB-Red) with package plasmids were contransfected into 293FT respectively, and the lentiviral viruses were harvested from 293FT. Viruses were used to infect liver cancer cell line (Huh-7) in tandem. We used doxcycline to induce the expression of target gene MST1 which was indentified by Western blot after 7-day cell culture. Results The recombinant inducable lentiviral vector containing MST1 gene was successfully constructed. The lentiviruses were also obtained and mediated by 293FT, which were highly efficient to infect liver cell lines Huh-7. The expression of MST1 was identified under Dox induction. Conclusions The recombinant inducable lentiviral vector containing MST1 gene has been successfully constructed. It is viable to obtain inducible cell lines Huh-7 with MST1 gene expression under Dox induction.
