Expression level of endogenetic nitric oxide on reproduction of human anterior cruciate ligament cells
- VernacularTitle:内源性一氧化氮在人前交叉韧带H( ACL)细胞增殖的表达水平
- Author:
Weiguo LIANG
;
Yan SHEN
;
Honghui CHEN
;
Yi TANG
;
Cancan ZHONG
- Publication Type:Journal Article
- Keywords:
Nitric oxide;
Anterior cruciate ligament cell;
Reproduction
- From:
Chinese Journal of Orthopaedic Trauma
2004;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression level of endogenetic nitric oxide(NO) in the reproduction process of human anterior cruciate ligament cells. Methods Anterior cruciate ligament cells were isolated and subcultured from the human anterior cruciate ligament. LPS was used to induce the anterior cruciate ligament cell to express the inducible nitric oxide synthase(iNOS ), and N-monomethyl -L-Arginine (L-NMMA) was used as the interdiction of nitric oxide. They were alone or together added in the culture medium of anterior cruciate ligament cells in different groups. The level of NO was indirectly measured in the medium of HACL. Results LPS promoted significantly anterior cruciate ligament cells to produce endogenetic nitric oxide, compared with the control group(P
