Protorlast Induced Mutation Breeding on Trichoderma aureoviride
- VernacularTitle:黄绿木霉原生质体诱变育种研究
- Author:
Qian YANG
- Publication Type:Journal Article
- Keywords:
Trichoderma aureoviride,Protoplast, Induced mutation, Breed
- From:
Microbiology
1992;0(04):-
- CountryChina
- Language:Chinese
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Abstract:
Based of the study on the impact of protoplasts under different conditions of enzymolysis solution, culture time, enzymolysis time and enzymolysis temperature in sequence using orthogonal test. The best optimal condition of producing protoplast was :0.5% helicase add 0.5% lysozyme and 1.0% cellulase, enzymolysis time was 3 hours , enzymolysis temperature was 32℃,under these condition the production of protoplast can be 4.25?10~(6 )per mL. Ca~(2+) and PEG can increase the regeneration ratio of protoplast.After induced mutation by complex factor under DES,UV and LiCl,the mutagenesis strain which own increasing enzyme activity and transmissibility steaty was obtained.
