The Production Influencing Factors of Extracellular Polysaccharide(EPS) from a Strain of Lactic Acid Bacteria and EPS Extraction
- VernacularTitle:一株乳酸菌胞外多糖产生的影响因素及其提取
- Author:
Ying CHEN
- Publication Type:Journal Article
- Keywords:
Lactic acid bacteria, Extracellular polysaccharide (EPS), Column chromatograghy, Thin layer chromatograghy
- From:
Microbiology
1992;0(04):-
- CountryChina
- Language:Chinese
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Abstract:
The influencing factors of EPS produced from LAB were studied by using the Phenol-H_(2)SO_(4 )method. It was demonstrated that the strain produced EPS at the most amount when it was incubated to 40~48h and pH4 under 30℃. Glucose is the most suitable sugar source for LAB producing EPS. The rough EPS was obtained from LAB' culture after centrifugation, dialysis, deprotein, decoloration, and ethanol-precipitation. The sample was at least composed of two polysaccharides that were different completely from the molecule weight and the amount. The purified EPS was got through the SephadexG-200 column and it was showed that it was a purified sample by thin layer chromatograghy.
