Using Gel Electrophoresis to Determine Lysogenic Bacillus thuringiensis
- VernacularTitle:用凝胶电泳方法鉴定苏云金芽孢杆菌溶源性菌株
- Author:
Quanping LUO
- Publication Type:Journal Article
- Keywords:
Bacillus thuringiensis , Phage, Lysogenicity
- From:
Microbiology
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Considering the feasibility that prophages could be induced, we used mitomycin C(MMC) to induce Bacillus thuringiensis (commonly referred to as Bt) strains used in this study . Firstly , the strains induced by MMC were centrifuged and the supernatants were treated with 2.5 SDS-EDTA. And then, they were put into the 0.7% agarose-gel electrophoresis to test whether there were DNA bands or not . The results of agarose-gel electrophoresis and double-layer plates indicated that the DNAs were from lysogenic Bts, not from no-lysogenic Bts. By using this method ,we could determine lysogenic Bts, and it is important for protecting Bts from phage infection during the process of fermentation .
