Transformation of Schistosomulae by Electroporation and Transient Expression of the Enhanced Green Fluorescent Protein (EGFP) Gene
- VernacularTitle:用电穿孔法将绿色荧光蛋白基因导入日本血吸虫童虫并表达
- Author:
Xiaosong YUAN
;
Jilong SHEN
;
Xuelong WANG
;
Yuansheng HU
;
Qingli LUO
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
Electroporation;
Fluorescent protein gene;
Transgenes;
Gene expression
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the possibility of heterogenous gene to express in juvenile Schistosoma japonicum and the application of electroporation in transformation of schistosomulae. Methods The plasmids of pEGFP-C1 were introduced into mechanically transformed schsitosomula with electroporation. The presence, transcription and translation of the transgene in electroporated schistosomula were confirmed by PCR, RT-PCR and Western blotting analysis respectively using the genomic DNA, total RNA and protein extracted and isolated from schistosomula cultured in vitro for 48 hours. Meanwhile, localization of EGFP within electroporated schistosomula was performed with confocal laser scanning microscope. Results 760 bp and 276 bp amplified products by PCR and RT-PCR were found coincident with the expected size and expression of EGFP gene in elctroporated schistosomula was confirmed by Western blotting. Fluorescence of EGFP was localized in tegument and subtegument of the electroporated schistosomula with confocal microscopy, especially in the anterior part of the worm. Conclusion The heterogenous gene of EGFP has been successfully introduced into juvenile S. japonicum by electroporation and the expression of transgene was confirmed with molecular and microscopical methods.
