Over-expression of Proapolipoprotein AI_(Milano) in Escherichia coli
- VernacularTitle:重组人载脂蛋白AI_(米兰变体)在大肠杆菌中的高效表达
- Author:
Mansheng DING
- Publication Type:Journal Article
- Keywords:
Proapolipoprotein AI,Milano,Inclusion body,Hydrophobic,Renaturation
- From:
Microbiology
1992;0(04):-
- CountryChina
- Language:Chinese
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Abstract:
To facilitate the mutation of Milano site, the whole proapo AI_(M) gene was divided to two fragments to synthesize by RT-PCR. The optimization of upstream gene sequence was carried out to improve proapo AI_(M) expression level in E. coli. After induction with a shift of temperature, yields of recombinant proapo AI_(M) achieved about 45 % of total cell protein and the recombinant proapo AI_(M) was expressed as a form of inclusion body in cells. The renaturation of protein was carried with a hydrophobic interaction column,the results showed that recombinant proapo AI_(M) had similar functional properties identical to those of native protein.
