Neuroprotective effect and its mechanism of estrogens in male Sprague-Dawley rats with cerebral ischemia
- VernacularTitle:雌激素对雄性大鼠缺血性脑损伤的神经保护作用及其可能机制
- Author:
Weimin YANG
;
Nong ZHOU
;
Kai WANG
- Publication Type:Journal Article
- Keywords:
estrogen;
cerebral ischemia;
neuroprotective effect
- From:
Journal of Clinical Neurology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the neuroprotective role and its mechanism of estrogens in male Sprague-Dawley rats with focal cerebral ischemia. Methods The rats were randomly divided into three groups: E2-pretreat group, E2+TAM (tamoxifen)-pretreat group and control group. The models of cerebral ischemia were maded by occlusion middle cerebral artery using an intraluminal filament method. 2 and 24 hours after focal cerebral ischemia, the neurological deficit scores and the death rate of rats were evaluated. 2,3,5-triphenyltetrazolium chloride (TTC) stain was used to assess the volume of infarction. Terminal transferase dUTP nick ending labeling (TUNEL) and immunohistochemistry were carried to observe neuron apoptosis and the expressions of Bcl-2, Bax and p53. Results Compared with control group, the volumes of infarction, death rates, neurological deficit scores and the numbers of neuron apoptosis both at 2 and 24 h were significantly decreased in E2-pretreated group (all (P
