Dynamic changes of nitric oxide content and caspase-3 mRNA expression in hippocampus in epileptic rats induced by pilocarpine
- VernacularTitle:匹罗卡品致大鼠海马一氧化氮水平和caspase-3 mRNA表达的动态变化
- Author:
Yingqi ZHANG
;
Weihong LIAO
;
Lin ZHENG
- Publication Type:Journal Article
- Keywords:
status epilepticus;
hippocampus;
caspase-3;
pilocarpine;
nitric oxide
- From:
Journal of Clinical Neurology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To probe the changes of nitric oxide (NO) content and caspase-3 mRNA expression in hippocampus and the correlation between NO and caspase-3 mRNA expression post-status epilepticus (SE). Methods The models of SE were induced by pilocarpine. The nitric oxide content was detected by chromatometry and caspase-3 mRNA expression was observed by reverse transcription polymerase chain reaction (RT-PCR) method. Results The level of NO increased rapidly at 6~72 h post-SE,and was still significantly higher than that of the controls (all (P)0.05).Conclusions The peak expression of nitric oxide is earlier than caspase-3 mRNA post-SE, although they have the similar diversity tendency. Nitric oxide pathway is definitely involved in the seizure initiation and correlated with caspase-3 activation.
