Study on the effect of triplex forming oligonucleotide on tumor growth and angiogenesis in rats with glioma
- VernacularTitle:三链形成寡核苷酸对大鼠脑胶质瘤生长和血管生成影响的研究
- Author:
Weifang LI
;
Dingbiao ZHOU
;
Xinguang YU
- Publication Type:Journal Article
- Keywords:
PDGF;
glioma;
VEGF;
angiogenesis
- From:
Journal of Clinical Neurology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the inhibit effect of triplex forming oligonucleotide (TFO)(platelet-derived growth factor-B chain, PDGF) on tumor growth and angiogenesis in rats with glioma.Methods 1?10~6 C_6 glioma cells with high-flow microinfusion were seed into right caudate putamens of 18 rats by stereotaxic technique. TFO was injected in situ 1 week after glioma cells inoculation. Treat group Ⅰ and treat group Ⅱ received TFO at dose of 1.5 mg/20 ?l and 3.0 mg/20 ?l, respectively. The same doses were given again at 8, 11 and 14th day after glioma cells inoculation. The control group was treated with 20 ?l normal saline at same time like treat groups. Three weeks after glioma cells inoculation, all the rats were killed. The expressions of, PDGF-B, vascular endothelial growth factor (VEGF) and proliferating cell nuclear antigen (PCNA) were detected with microscopic histology.Results The inhibition rate of tumor growth was 66.0% in treat groupⅠand 92.2% in treat group Ⅱ. There was significant difference between the two groups ((P
