The application of IS6110 restriction fragment length polymorphism and spoligotyping in identification of Mycobacterium tuberculosis strains
- VernacularTitle:IS6110限制性片段长度多态性和间隔区寡核苷酸分型技术在结核分枝杆菌菌株鉴定中的应用
- Author:
Yanling GUO
;
Sumin WANG
;
Chuanyou LI
;
Yang LIU
- Publication Type:Journal Article
- Keywords:
Polymorphism, restriction fragment length(RFLP);
Nucleotides;
Mycobacterium tuberculosis
- From:
Chinese Journal of Laboratory Medicine
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the application of IS6110-restriction fragment length polymorphism (RFLP)and Spoligotyping in the epidemiology of tuberculosis and to discuss the characteristic of the Mycobacterium tuberculosis in different regions in China.Methods 158 isolates of Mycobacterium tuberculosis strains, were verified by IS6110-RFLP and spoligotyping.Results The discriminatory power of IS6110-RFLP was higher than that of spoligotyping. The result of spoligotyping was compared with the international spoligotype database. Fourteen types belonged to the shared types, in which the type 1 was epidemic and widely,called Beijing genotype. There was significantly difference among the mycobacterium tuberculosis between Guangdong and other regions in clustered rate and the proportion of Beijing genotype (P
