Modulation of T-lymphocyte subsets production with imiquimod in the PBLN cell cultures from ovalbumin-sensitized rats
- VernacularTitle:咪喹莫特对致敏大鼠支气管旁淋巴结T辅助淋巴细胞亚群产生细胞因子的影响
- Author:
Qiaozhen WU
;
Kaisheng YIN
;
Xiang WANG
- Publication Type:Journal Article
- Keywords:
Imiquimod;
Lymph nodes;
Lymphocyte subsets
- From:
Chinese Journal of Laboratory Medicine
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect and mechanism of imiquimod on T helper (Th) cell subsets in the Parabronchial lymph node (PBLN) cell cultures from ovalbumin (OVA)-sensitized rats.Methods PBLN were isolated and cultured. PBLN cells were divided into A~F, according to different concentrations of intervention. Cultured for 0, 3, 6, 12, 24, 48 hours, the expressions of IL-4 and IFN-? in supernatants were determined by ELISA. The mRNA expressions of the cytokines in cells were detected by RT-PCR.Results In the group A, only low concentrations of IFN-? were detected. Based on the cultured time, the concentrations of IFN-? were increased significantly if imiquimod concentration was between 1 and 10 ?g/ml. Levels of IL-4 were increased slowly compared with those in the group B (P0.05).Conclusion Imiquimod show the best effect on antigen-specific Th cell subsets when cultured for 12h. The results suggest that imiquimod have benefit in atopic diseases such as the late inflammation reaction of asthma.
