Influence of trastuzumab with IL-2 on HER2 and MRP1 of ACHN
- VernacularTitle:人表皮生长因子受体2抑制剂联合白细胞介素-2对人肾癌细胞株的疗效观察
- Author:
Jiajing WANG
;
Zhiquan HU
;
Zhangqun YE
- Publication Type:Journal Article
- Keywords:
Human caucasian kidney adenocarcinoma;
Interleukin-2;
Human epidermal growth factor receptor2;
Multidrug resistance-associated protein 1
- From:
Journal of Medical Postgraduates
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the influences of Trastuzumab and IL-2 on human epidermal growth factor receptor-2 (HER2), multidrug resistance-associated protein1 (MRP1) of ACHN in vitro . Methods: ACHN cell line of RCC were cultured by cell culture technique. The tetrazolium-based colorimetric assay was used to evaluate the growth inhibitory effects of trastuzumab and IL-2. S-P method was used to determine the expression of HER2, MRP1 of the cells. Results: Trastuzumab showed the inhibitory effects on growth and multi-drug resistance in RCC from 40 ?g/L or 24 h in time-effective and dose-dependent manner. After treatment with Trastuzumab and/or IL-2, the expression of HER2, MRP1 genes of RCC was decreased significantly( P
