A convenient and fast method to amplify DNA fragments from filamentous fungi
- VernacularTitle:丝状真菌脱氧核糖核酸片段快速扩增法
- Author:
Liqing LI
;
Mingquan SU
;
Xiaoke HAO
- Publication Type:Journal Article
- Keywords:
Filamentous fungi;
PCR;
DNA
- From:
Journal of Medical Postgraduates
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Using PCR to amplify filamentous fungi DNA fragments. Methods:Pour some liquid nitrogen to the hypha and grind them to powder. Then add some TE buffer and boil them for 15-20 minutes. Take a little supernatant as PCR template. After PCR do agarose gel electrophoresis. Collect the gel containing the objective fragment and add some TE. Take a little supernatant as PCR template (again.) After PCR, do agorose gel electrophoresis again. Results:We obtained a great deal of objective DNA. Conclusion:Amplifying filamentous fungi DNA fragment by this way, we can save time and money. We can also improve our work efficiently.
