The use of autologous supernatant of malignant pleura effusion in culturing human tumor infiltrating lymphocytes in vitro
- VernacularTitle:自体胸腔积液上清作为培养液对肿瘤浸润淋巴细胞生长的影响
- Author:
Zhengyun ZOU
;
Baorui LIU
- Publication Type:Journal Article
- Keywords:
Culture medium;
Malignant pleura fluid;
Tumor infiltrating lymphocyte;
Tumor immunotherapy
- From:
Journal of Medical Postgraduates
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of autologous supernatant of malignant pleura effusion and RPMI1640 with 10% AB+ serum on the culture of tumor infiltrating lymphocytes (TIL) in vitro. Methods:Isolated by the attachment method we established, TIL was cultured in either autologous supernatant of malignant pleura fluid or RPMI1640 with 10% AB+ serum, and various types of cytokines such as (IL-2,) PHA and antibody against CD3 (OKT3) were added into both cultures. The proliferation as well as the killing activity and the phenotype changes of TIL cultured in the two kinds of cultures were compared. Results:There was no difference in the proliferation or the killing activity in vitro as well as the phenotype changes of TIL between the two kinds of cultures. Conclusion: Autologous supernatant of malignant pleura fluid could be used as TIL culture medium. The method described here made it possible for TIL to be cultured in vitro for a short time, and then reinfused back to thorax for further expanding and controlling the malignant pleura effusion in the presence of IL-2, and it alsominimized the risks of contamination.
