Study on preparation of mucopolysaccharide from Dasyatis akajei cartilage
- VernacularTitle:赤魟软骨黏多糖的制备
- Author:
Hongyu LUO
;
Peng GAO
;
Ru SONG
- Publication Type:Journal Article
- Keywords:
Dasyatis akajei cartilage;
mucopolysaccharide;
alkali extracting;
enzymolysis
- From:
Chinese Journal of Marine Drugs
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
The technique of extracting and refining mucopolysaccharide from Dasyatis akajei cartilage obtained was studied and the content of mucopolysaccharide was measured.The results showed that when the content of NaOH was 6%,temperature 40℃, extracting time 6h,followed neutral protein enzyme dissolution for 7h under 40℃,protein precipitation with trichloroacetic acid and 4 times volume 95% C_(2)H_(5)OH for polysaccharide precipitation.the extraction rate was over 21%, the product was white powder and its purity was about (84.53%).Physical and chemical properties of the mucopolysaccharide were identified.