Synthesis of lactosaminated carboxymethylchitosan and its action of plasmid DNA delivery
- VernacularTitle:乳糖化羧甲基壳聚糖的合成及转运质粒DNA的作用
- Author:
Xuedong WANG
- Publication Type:Journal Article
- Keywords:
lactosaminated carboxymethylchitosan;
synthesis;
DNA;
delivery
- From:
Chinese Journal of Marine Drugs
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the synthesis of lactosaminated carboxymethylchitosan (Lac-CMCS) and its action of delivering plasmid DNA into cells. Methods The lactosaminated carboxymethylchitosan was synthesized by reductive amination and was conjugated with plasmid contained antisense x gene. This complex was delivered into cells and these cells were cultured in 37℃,5%CO_(2). Testing the inhibition on HBV by determining HBsAg and HBeAg produced by HepG2.2.15 cells with ELISA technique. Results The molar ratio between lactose and CMCS was 15∶1 in this covalence conjugate. The best mass ratio of DNA to Lac-CMCS was 1∶10 in DNA/Lac-CMCS which was able to deliver gene into cells, and the ma-ximal inhibition rate was 83.6%. Conclusion In vitro, the lactosaminated carboxymethylchitosan can effectively deliver DNA into cells.
