Spectrophotometric determination of chondroitin sulfate with 4-amino-3-hydrazine-5-mercapto-1,2,4-triazole
- VernacularTitle:AHMT分光光度法测定软骨鱼类硫酸软骨素
- Author:
Kun LIU
;
Hua GAO
- Publication Type:Journal Article
- Keywords:
chondroitin sulfate(ChS);
spectrophotometry;
4-amino-3-hydrazino-5-mercapto-1,2,4-triazole
- From:
Chinese Journal of Marine Drugs
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
To establish a new method for the determination of chondroitin sulfate. The method was based on the interaction of sodium chondroitin sulfate with 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole to yield a violet product, 6-mercapto-3-substituted-s-triazolo-\[4,3-b\]-s-tetrzaine derivatives. Absorbance at 540 nm obeyed Beer's Law within the concentration range from 0.1 to 1.0 g?L~(-1)and the results were precise and accurate. The method was operationally simple and had a good precision and sensitivity.
