Advances in vascularization of tissue-engineered graft
- VernacularTitle:组织工程组织血管化研究新进展
- Author:
Qunli WANG
;
Guoxian PEI
- Publication Type:Journal Article
- Keywords:
Tissue engineering;
Vascularization
- From:
Chinese Journal of Orthopaedic Trauma
2004;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Vascularization is one of the major c hallenges in tissue engineering gra ft.Vascular plexus falls into two modes which are different in recourse of endotheliocyte:vascul ogenesis and angiogenesis.Both mod es are a dynamic and complicated physiological process.Various kinds of growth factor and adhesion molecule modula te the process.Both in vitro and in vivo methods are used in vascularization research.In vitro methods are used to e-valuate biomaterials for endothelial cell attachment,cytotoxicity,g rowth,angiogenesis and its effects on gene reg-ulation.Chorioallantoic membrane(CAM)is one of the simplest and effective models in vivo.Animals are used as more advanced models.The strategie s of vascularization of tissue engin eering include:modification of the surface of the biomaterials,addition of slow-release growth factor to scaffold,e ndotheliocyte cocultured with other seed cells,wrapping by vascular net,implantin g blood vessel bundles into the graft,seeding biomaterial implants with vascular remnants as vascular template,and c reating perfused microvessels in vitro.None of these approaches,however,is satisfactory at present.We believe that an ideal method will be finally f ound for vascularization in tissue e ngineering with advancement of research on mech anism of vascularization and progre ss in techniques.[
