Measurement of Morphological Parameters of Cryptosporidium murisOocysts by Digital Image Processing Technology
- VernacularTitle:数字图像处理技术对隐孢子虫卵囊形态学参数的测定
- Author:
Yuyan BING
;
Yan LI
;
Chunli AN
- Publication Type:Journal Article
- Keywords:
Cryptosporidium muris;
Oocyst;
Digital image processing;
Morphological parameter
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish and analyze the morphological parameters of the oocysts of Cryptosporidium muris for defining their morphological change. Methods Oocysts were collected from KM mice(immunodepressed by dexamethasone for 10 days) and examined with modified acid-fast staining. Images of 1 190 oocysts were acquired by photograph system. The length, width, perimeter, area and equivalent diameter of the oocysts were obtained by computer digital image processing system and analyzed by SPSS software (Version 11.0). Result The average length of the oocysts was 5.93 ?m, ranging from 3.36 ?m to 8.51 ?m in 95% confidence interval of them. The average width was 4.96 ?m, ranging from 3.26 ?m to 6.66 ?m in 95% confidence interval of them. The average perimeter was 18.03 ?m and the average area was 16.08 ?m2. Conclusion Data obtained from the computer system are objective and precise, offering scientific foundation for measuring the oocysts and for identifying Cryptosporidium spp.[
