The effect of Tongxinluo on the apoptosis of smooth muscle cell and the protein expressions of the Bcl-2 and Bax after angioplasty in rabbits
- VernacularTitle:通心络对家兔血管成形术后平滑肌细胞凋亡及Bcl-2和Bax蛋白表达的影响
- Author:
Dongmei LIU
;
Guinan LIU
;
Dongyun HAN
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Tongxinluo;
Angioplasty, balloon;
Proto-oncogene proteins
- From:
Chinese Journal of Interventional Cardiology
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Tongxinluo on VSMC apoptosis and protein expression of Bcl-2 and Bax in rabbits after angioplasty. Methods Atherosclerotic and PTCA models were set up in 60 rabbits which were randomly divided into 3 groups, the control group the hypertipidemia group and the Tongxinluo group. Apoptosis was determined by TUNEL method and protein expressions of Bcl-2 and Bax were detected by S-P immunohistory chemistry staining. Results Apoptosis of VSMC in the hyperlipidemia group was less than that in the control group, but the level of apoptosis in the Tongxinluo group was even higher compared with the hyperlipidemia group. The level of Bcl-2 expression was found higher in the hyperlipodemia group than in the control group. The expression was mainly seen in endothelium and tunica media of the arteries. The expression of Bcl-2 in the Tongxinluo group is the least among the 3 groups studied. Bax expression, which can mainly be seen in the endothelia, is higher in the Tongxinluo group than that in the hyperlipidemia group. Conclusion Tongxinluo may enhance the apoptosis of VSMC, downregulating the expression of Bcl-2 protein and upregulating the expression of Bax protein.
