Detection of fetal RhCcEe genotype in maternal plasma
- VernacularTitle:从孕妇血浆中提取胎儿DNA鉴定胎儿RhCcEe血型
- Author:
Ji HE
;
Faming ZHU
;
Xiaozhen HONG
;
Jinhui LIU
;
Shu CHEN
;
Fei QIN
;
Lixing YAN
- Publication Type:Journal Article
- Keywords:
Blood groups;
Prenatal diagnosis;
Fetal DNA
- From:
Chinese Journal of Laboratory Medicine
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect fetal RhCcEe genotype from fetal DNA in maternal plasma for noninvasive prenatal diagnosis.Methods DNA from maternal plasma sample was extracted by use of QIAamp DNA Kit. The existence of fetal DNA was confirmed by amplified fetal SRY gene. The fetal RhCcEe gene was amplified by polymerase chain reaction (PCR) from 30 pregnant maternal plasma. The results of fetal RhCcEe genotype were evaluated retrospectively by the serologic analysis of infant and pregnant woman RhCcEe phenotype.Results Among the 30 samples, 13 were the same phenotypes between mother and infant, 17 were different. When mother phenotypes were RhCC, cc, EE and ee homozygous, the deleted allele gene can be successfully amplified from mother plasma.Conclusion Noninvasive fetal RhCcEe genotyping is reliable. When the mother was homogyzous, genotyping the fetal CcEe alleles was very significant and useful for HDN (hemolysis disease of newborn) diagnosis and therapy.
