Antioxidative effect of polypeptide from Chlamys farreri on HaCaT cells damaged by ultraviolet B
- VernacularTitle:扇贝多肽对UVB损伤HaCaT细胞的抗氧化作用
- Author:
Mianqing HUANG
;
Chunling YAN
;
Mei DOU
;
Songmei ZHAN
;
Meizhi WANG
;
Yuejun WANG
;
Chunb WANG
- Publication Type:Journal Article
- Keywords:
polypeptide from Chlamys farreri;
ultraviolet B;
antioxidation
- From:
Chinese Journal of Marine Drugs
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the antioxidative effect of polypeptide from Chlamys farreri (PCF) on HaCaT cells damaged by ultraviolet B (UVB). Methods HaCaT cells model established by UVB irradiation were randomly divided into seven groups. After pretreatment with different concentrations of PCF for 1 h, the cells were irradiated by UVB at a dose of 30mJ/cm~ 2, and followed further incubatiou for 18 h. Enzyme activities including superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) were determined by biochemical methods. Total antioxidative capacity (T-AOC) and malondialdehyde (MDA) were also determined. Results PCF could enhance the activities of SOD, GSH-Px and CAT; increased T-AOC and inhibited MDA formation. Conclusion These results indicated that PCF exhibited protective effects on HaCaT cells irradiated by UVB owing to its antioxidative action.
