The effects of hyperthermia on apoptosis in human colonic carcinoma cell line Lovo
- VernacularTitle:加热诱导结肠癌细胞Lovo凋亡的研究
- Author:
Huiming ZHU
;
Na WANG
;
Xun HUANG
- Publication Type:Journal Article
- Keywords:
Hyperthermia;
Human colonic cacinoma cell;
Apoptosis
- From:
Journal of Medical Postgraduates
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of hyperthermia on apoptosis in human colonic cacinoma cell line Lovo. Methods:Lovo cells were exposed to hyperthemia and apoptosis was measured with fluorescent Hocehst 33258 staining, electropherogram gel, flow cytometry (FCM). Results:Significant changes in cell morphology , apoptosis peak apeared before the G1 phase and DNA ladder was observed in various group cells which were exposed to different temperature and in different time. Most significent changes were observed in cells exposed to 43℃ for 3,4 h and 44℃ for 1,2 h. Also cell cycle was found changed. Conclusion:Hyperthermia can cause apoptosis in human colonic cacinoma cell line Lovo, and has phase-specificity.
