Effects of adenoviral vector mediated rat AT2R gene transfer on intima proliferation after rat carotid artery injury
- VernacularTitle:大鼠血管紧张素Ⅱ2型受体基因转染对颈动脉球囊损伤后新生内膜形成的影响
- Author:
Bing TANG
;
Guoxiang HE
;
De LI
- Publication Type:Journal Article
- Keywords:
Receptors, angiotensin;
Tunica intimal;
Gene therapy
- From:
Chinese Journal of Interventional Cardiology
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of rat angiotensin Ⅱ receptor (AT2R) gene transfer mediated by adenoviral vector on neointimal proliferation after rat carotid artery injury. Methods AT2R gene was transducted into the carotid artery by adenoviral vector with GFP after carotid balloon injury. Restenosis models were established in Wistar rats. Immunostaining was performed to examin the expression of AT2R in local arteries. Neointima/media area ratio at day 21 after gene transfer was measured by morphometric analysis. Results The transduction rate of AT2R gene in rat carotid arteries was 40% and the gene expressed constantly in the vessel walls. Neointima/media area ratio of the gene transduction group was significantly reduced (48%) compared with the control group at day 21 after gene transfer. Conclusion The results suggest the possibility that AT2R gene transfer can inhibit proliferation of arterial intima after balloon injury and it is a potential therapeutic approach to prevent neointimal hyperplasia.
