Genetic analysis of human immunodeficiency virus type 1 strains from AIDS patients in Shenzhen
- VernacularTitle:逆转录聚合酶链反应在艾滋病患者HIV-1毒株env基因的序列测定和亚型分析中的应用
- Author:
Hui WANG
;
Bing LI
;
Liumei XU
;
Jian LU
;
Lixiong LI
;
Yiwen HU
;
Boping ZHOU
- Publication Type:Journal Article
- Keywords:
Reverse transcriptase polymerase chain reaction;
HIV-1;
Genes;
Sequence analysis
- From:
Chinese Journal of Laboratory Medicine
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify subtypes of human immunodeficiency virus 1(HIV-1)strains from the AIDS patients in Shenzhen and determine whether the HIV-1 subtypes differ in disease progression.Methods HIV-1 env gene was amplified by the nest-RT-PCR from plasma obtained from 26 patients with AIDS in Shenzhen. The C2-V3 regions were sequenced to identify subtypes The plasma viral loads and CD4T lymphocyte were measured as the same time.Results Phylogenetic trees showed that the 12 AIDS patients had subtype B in which, one was close with the U.S reference strain and 11 with the Chinese Yunnan reference strain;13 AIDS patients had subtype CRF01-AE from Thailand;There were no differences in the CD4 cell count and plasma HIV-RNA levels between individuals infected with subtypes B and CRF01-AE.Conclusion Our study indicated that HIV-1 subtype B and CRF01-AE strains were present in AIDS patients in Shenzhen. There was no evidence that the subtypes of virus could determine disease progression.
