Expression and identify of CH50 polypeptide in bladder cancer cell line BIU-87
- VernacularTitle:CH50多肽在膀胱癌细胞系BIU-87中的表达和鉴定
- Author:
Zhuang WU
;
Zhong CHEN
;
Zhangqun YE
;
Shiqia YE
- Publication Type:Journal Article
- Keywords:
CH50 polypeptide;
BIU-87 cells;
Gene transfection
- From:
Journal of Medical Postgraduates
2003;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the expression and identification of CH50 polypeptide in bladder cancer cell line BIU-87 by gene transfection. Methods:The eukaryotic expressing vector pCH510 of polypeptide CH50 was introduced into BIU-87 cells by gene transfection Lipofectimine TM2000.The expressed product was identified by immunohistochemistry and Western blot method. The expression of the transfected gene was identified by RT-PCR. Results:The BIU-87 cells could produce CH50 polypeptide by transfection. Conclusion:When the vector of pCH510 was transfected into BIU-87 cells in vitro, the cell adhension characteristic would be changed.
