Inhibitory effect of phorbol 12-myristate 13-acetate and angiotensin-(1-7) on angiotensin Ⅱmediated proliferation and secretion of cultured rat glomerular mesangial cells
- VernacularTitle:血管紧张素-(1-7)和佛波醇酯拮抗血管紧张素Ⅱ诱导的大鼠系膜细胞增殖及分泌
- Author:
Lianhuan MA
;
Jian LIU
;
Mingyong WANG
;
Hongya HE
- Publication Type:Journal Article
- Keywords:
Angiotensin-(1-7);
Phorbol 12-myristute 13-acetate;
AngiotensinⅡ;
Cell proliferation;
Extracellular matrix
- From:
Journal of Medical Postgraduates
2003;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the influence of angiotensin-(1-7) \[Ang-(1-7)\] and phorbol 12-myristate 13-acetate (PMA)on the proliferation and secretion of cultured rat glomerular mesangial cells (GMC) induced by angiotensinⅡ(AngⅡ). Methods: Ang-(1-7) and PMA was used in cultured rat glomerular mesangial cells induced by AngⅡ, synthesis of DNA and protein, change of cell number were observed for rat GMC proliferation. Secretion of PcⅢ and HA was measured by radioimmunoassay in culture medium of rat GMC. Results: Ang-(1-7) and PMA both inhibited the AngⅡinduced synthesis of DNA and protein, increase of cell number, and secretion of PcⅢ and HA in cultured rat GMC. Conclusion: Ang-(1-7) and PMA both can inhibit the AngⅡ induced proliferation and secretion of cultured rat GMC.
