Conotoxin RNA isolation and its cDNA synthesis
- VernacularTitle:芋螺毒素RNA的提取及其cDNA合成的研究
- Author:
Yaru QUAN
;
Sulan LUO
;
Qiujin LIN
;
Dongting ZHANGSUN
;
Ben ZHANG
- Publication Type:Journal Article
- Keywords:
RNA extraction;
Conus;
cDNA synthesis
- From:
Chinese Journal of Marine Drugs
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Two methods of different RNA extractions from Conus venom tube and toxin gland of seven cone snail species were performed. ThermoScript~ TM RNase H~ - Transcriptase and AMV Transcriptase were used to synthesize cDNA separately. The cDNAs were used as templates to amplify conotoxin genes by PCR.The optimized RNA isolation and cDNA synthesis methods were obtained according to RT-PCR results, which would be the basis for cloning of new conotoxin genes.
