Inhibitory effect of arsenic trioxide on the activity of transgenic tumor necrosis factor-? promoter in vascular smooth muscle cells and macrophages
- VernacularTitle:三氧化二砷抑制血管平滑肌细胞及巨噬细胞中转染的肿瘤坏死因子-?启动子活性
- Author:
Zhuoqi ZHANG
- Publication Type:Journal Article
- Keywords:
Arsenic trioxide;
TNF-?;
promoter;
vascular smooth muscle cells;
macrophages
- From:
Basic & Clinical Medicine
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of arsenic trioxide(As_(2)O_(3)) on the transgenic TNF-? promoter activity in cultured vascular smooth muscle cells(VSMCs) and macrophages(M?s).Methods Human TNF-? promoter was constructed by reporter gene system and was transiently transfected into VSMCs and M?s.Promoter activity was tested by luciferase activity with or without LPS and Ang Ⅱ stimulation,before or after different dosage of As_(2)O_(3) treatment.Results The results showed: TNF-? promoter stably expressed in VSMCs and M?s compared with CMV promoter(58.3% and 55.6%,respectively).Both LPS and Ang Ⅱ up-regulated TNF-? promoter activity (P
