Effects of chitosan on proliferation and differentiation of human periodontal ligament cells
- VernacularTitle:甲壳胺对人牙周膜细胞增殖和分化的影响
- Author:
Zhao MAO
;
Jian YANG
;
Xi MAO
;
Ha SU
- Publication Type:Journal Article
- Keywords:
human periodontal ligament cells;
chitosan;
proliferation;
differentiation;
alkaline phosphatase;
osteocalcin
- From:
Chinese Journal of Marine Drugs
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe effects of chitosan on proliferation and differentiation of human periodontal ligament cells( PDLC). Methods The human periodontal ligament cells were isolated and cultured.The effects of chitosan on proliferative ability of human PDLCs were evaluated with MTT method, and alkaline phosphatase activity and the secretion of osteocalcin of human PDLCs were measured with spectrophotometric assay and radioimmunological method. Results Compared with control group, the proliferative ability of human PDLCs in Chi(0.05g?L~ -1) group and Chi(0.1g?L~ -1) group at 3,5,7 day was considerably increased (P
