Isolation, Purification and its antitumor activity of glycosaminoglycan from Pinctada martensii
- VernacularTitle:珠母贝氨基多糖的分离纯化及其抗肿瘤活性的初步研究
- Author:
Xiuping FAN
;
Hongmian WU
;
Xiaoling LEI
;
Xueqiong HU
- Publication Type:Journal Article
- Keywords:
Pinctada martensis;
glycosaminoglycan(GAG);
chromatography purification method;
antitumor activity
- From:
Chinese Journal of Marine Drugs
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Crude glycosaminoglycan(CPG) from the whole viscera of Pinctada martensii was isolated with the two-enzyme hydrolysis and purified by the chromatograph.And the antitumor activity was studied with MTT colormetric assay. The CPG could be separated into two fractions(GAG-1, GAG-2) by the DEAE-52-cellulose ion exchange chromatograph. The contents of hexosamine of CPG,GAG-1, GAG-2 were 54.6%,50.0%,35.4%,respectively. The sulfate of GAG-2 was 13.8%. The inhibiting rates of CPG, GAG-1 and GAG-2 to HL-60 cells were 54.6%, 50.0% and 35.4%,respectively. The inhibition to HL-60 of 5-Fu could be increased by combined use with CPG, GAG-1 and GAG-2, GAG-1 was the main active fraction.
