Effects of glycosaminoglycan from scallop skirt on foam cell formation and its function
- VernacularTitle:扇贝糖胺聚糖对平滑肌源性泡沫细胞形成及其功能的影响
- Author:
Fusheng SUN
;
Sai LIU
- Publication Type:Journal Article
- Keywords:
Glycosaminoglycan from Scallop Skirt;
Foam cell,smooth muscle ceell-derived;
Nitric oxide;
Atherosclerosis
- From:
Journal of Medical Postgraduates
2003;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of glycosaminoglycan from scallop skirt (SS-GAG) on the formation of foam cells from porcine artery smooth muscle cells (SMCs) and the expression of the total superoxide dismutase(SOD)and glutathione peroxidase(GSH-PX)activity, NO production and the mechanism of anti-atherosclerosis action of SS-GAG. Methods: SMCs were incubated with 15 mg/L oxidized low-density lipoprotein (Ox-LDL) for 72 h to establish a smooth muscle cell-derived foam cell model. In addition, SMC cells were divided into 6 groups:①blank group,②model(Ox-LDL) group, ③Ox-LDL+200 mg/L SS-GAG group,④Ox-LDL+400 mg/L SS-GAG group,⑤Ox-LDL+800 mg/L SS-GAG group, ⑥Ox-LDL+Heparin 100 mg/L group. After 72 h incubation, intracellular total cholesterol (TC), free cholesterol (FC), cholesteryl ester (CE) content and CE/TC ratio were measured through enzymatic method. The SOD, GSH-PX and NO concentration in the medium were also determined through Xanthine oxidase method or TBARs. Results: TC, CE, CE/TC in model group significantly increased, while FC, GSH-PX and NO concentration in the medium significantly decreased compared with blank group. After treatment with heparin (100 mg/L) and different concentrations of SS-GAG (200 mg/L, 400 mg/L,800 mg/L), TC, CE, and CE/TC significantly decreased (P
