Gentamicin-Chitosan gel for drug delivery system in vitreous of rabbit eyes
- VernacularTitle:医用几丁糖庆大霉素玻璃体内缓释的毒理研究
- Author:
Qing WEI
;
Xiaofang ZHANG
;
Jinsong ZHANG
- Publication Type:Journal Article
- Keywords:
Chitosan;
Sustained release;
Vitrious;
Toxicity
- From:
Journal of Medical Postgraduates
2003;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the retinal toxicity of chitosan and gentamicin- chitosan gel. Methods:Sixteen albino New Zealand rabbits (weighted 1. 5-2.5 kg) were used in this study. The rabbits were divided into 4 groups randomly;each group was injected with 0.1 ml PBS, gentamicin 2 mg/ml,3% Chitosan gel, and gentamicin-chitosan gel (2 mg/ml) respectively. ①Ophtahlmoscopic examinations were done. Retina was observed prior to injection , and on 1st, 3 rd, 7 th, 14 th d and 28 th d after injection with direct ophthalmolscope observations. ②Electroretinographs (ERGs) were recorded prior to injection, and then on 3 rd,7 th,14 th d and 28 th d after injection were carried out. ③Light microscope and transmission electronicmicroscope examinations were performed four weeks after the injection. All eyes were enucleated, the retina examples were observed under light microscope and transmission electronic microscope. Results:Intravitreous injection with 3% chitosan was free of retina toxicity. The retina toxicity effects of gentamicin 200 ?g could be found with transmission electron microscope, but gentamicin-chitosan gel with the same dosage could decrease the damage. Conclusion:The result shows that chitosan can be used in the intravitreous injection.
