Metagenome Cloning—A New Approach for Novel Microbial Bioactive Compounds Discovery
- VernacularTitle:宏基因组克隆——微生物活性物质筛选的新途径
- Author:
Bing YAN
- Publication Type:Journal Article
- Keywords:
Metagenome, Environmental total DNA, Function-driven screening, Sequence-driven screening
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
It is reported that more than 95% microbe species non-cultured or un-cultured yet in now days by the traditional cultivation approach, which limits discovering novel bioactive compounds from microorganisms. ‘Metagenome’ is the genomes of the total microbiota found in nature. Metagenome libraries were constructed by directly extracting DNA from environmental sample and transforming to surrogate host. The libraries were screened for novel bioactive compounds or genes surrounding their synthase in different strategies of function-driven or sequence-driven. These have enormously amplified the space of microbial resource utilization and enhanced the opportunity of obtain novel bioactive compounds.
