The effects of methotrexate on the neointimal formation after balloon injured arteries in rabbits
- VernacularTitle:甲氨蝶呤抑制兔颈动脉球囊损伤后新生内膜形成
- Author:
Lei MENG
;
Wei GAO
;
Zhuo YU
- Publication Type:Journal Article
- Keywords:
Restenosis;
Methotrexate;
Endothelium, vascular
- From:
Chinese Journal of Interventional Cardiology
1996;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of methotrexate on the neointimal formation after arterial balloon injury. Methods Male rabbits were randomized into four groups: MTX 0.5 mg/kg per week, MTX 1.5 mg/kg per week, MTX 5 mg/kg per week and the control. Drugs were administered by intramuscular injection. Rabbit carotid arteries were harvested 2 and 4 weeks after injury. Results Histologically, the intimal areas were reduced significantly in MTX treated animals compared with the controls. The VSMC proliferation in injured vessels was identified by immunostaining for proliferating cell nuclear antigen (PCNA). In comparison, PCNA-positive cells in both intima and media were significantly reduced by treatment of MTX. But MTX did not enhance reendothelialization in the injured carotid arteries as determined by Evans blue stain.Conclusion Low dosage of MTX could attenuate neointimal formation after arterial injury by inhibiting VSMC proliferation.
