Neuronal injury induced by platelet activating factor involved in NMDA/PSD_(93) signaling pathway
- VernacularTitle:血小板活化因子通过N-甲基-D-天(门)冬氨酸/突触后密度蛋白93途径致神经元损伤
- Author:
Yun XU
;
Lan WANG
;
Rong HUANG
- Publication Type:Journal Article
- Keywords:
platelet activating factor;
cortex neuron;
NMDA/PSD 93 signaling pathway
- From:
Journal of Clinical Neurology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study whether neuronal injury induced by platelet activating factor (PAF) was involved in NMDA /PSD 93 signaling pathway.Methods Primary cortex neurons culture were from wild type and PSD 93 knockout mice.Then the neurons were pretreated with 0.3 ?mol/L PAF for 24 hours, 5 ?mol/L BN52021, 10 ?mol/L MK-801, or 60 ?mol/L L-NAMA, respectively. The cells were stained with PI/Calcein for apoptosis detection. Varied protein expressions were also observed in the neurons by western blot. Proteins colocalized on neuraxon were detected by cell immunochemistry and confocol microscopy, and cGMP activity was measured by radioimmunoassay.Results (1) PSD 95, NR2A, and nNos except PSD 93 expressed in the cortex neurons from PSD 93 knockout mice. (2) PSD 93, NR2A and nNos colocalized on neurite. (3) Neurotoxicity and cGMP avtivity induced by PAF decreased in PSD 93 knockout cortex neurons.Conclusion NMDA/PSD 93 signaling pathway is involved in neuronal injury induced by PAF.
