Experimental study on osteogenic differentiation of allogeneic bone marrow stromal cells in muscles of rabbits
- VernacularTitle:同种异体骨髓基质细胞肌肉内成骨分化的实验研究
- Author:
Chenglin YANG
;
Zhenggang BI
;
Yuxue WANG
;
Quan QI
- Publication Type:Journal Article
- Keywords:
Bone marrow stromal cells;
Allogeneic;
Bone;
Tissue engineering;
Transplantation
- From:
Chinese Journal of Microsurgery
2008;31(3):203-206,illust 3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study and evaluate the ability of allogeneic bone marrow stromal cells(BMSCs) to survive and regenerate bone in muscles without using immunosuppressive agents. Methods A complete mismatch between donor BMSCs and recipient rabbit was confirmed by one way mixed lymphocyte reaction assaysprior to implantation. And then bone marrow aspirates were obtained from donor rabbits.BMSCs were isolated from the bone marrow, cultured in conditional medium to be ubdyced to become osteogenic, and then to examine these characteristics. After that the donor BMSCs were transplanted into the recipient rabbits. Immunological tests such as lymphocyte transformation rate and cell mediated cytotoxity, histological observation, seeding cells survival and bone formation were performed following transplantation. Results Allogeneic BMSCs transplantation did not actually elicit an adverse immune response, and bone regenerated at the transplantation area, and then the transplanted BMSCs marked with BRDU preoperatively were founded to be living 8 weeks later after transplantation using immunohistochemistric technique. Conclusion Allogeneic BMSCs would not elicit an adverse immune response in vivo without the immunosuppressive therapy, which could survive and form new bone tissue with the help of BMP-2 in the muscles.
