Study on the genes for TEM and aminoglycoside-modifying enzymes
- VernacularTitle:鲍曼不动杆菌TEM基因及氨基糖苷类修饰酶基因初步研究
- Author:
Yueqing ZHOU
;
Kailai LU
;
Zuhuang MI
- Publication Type:Journal Article
- Keywords:
Acinetobacter;
beta-Lactamases;
Aminoglycosides;
Genes
- From:
Chinese Journal of Laboratory Medicine
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
The genes for TEM and aminoglycoside-modifying enzymes (AMEs) in Acinetobacter baumannii were studied to provide molecular biological support for monitoring their drug resistance.MethodsTwenty strains of Acinetobacter baumannii were clinically isolated from the period of August, 2002 to Feburary, 2004. The drug resistance of these strains to thirteen kinds of antimicrobial agents was tested by microdilute test. The TEM and AMEs genotypes of these strains were analyzed by polymerase chain reaction and verificated by DNA sequencing .Results All twenty strains were sensitive to Imipenam and Meropenem. The resistant rates of the twenty strains to Piperacillin/ Tazobactam, cefepime and ceftazidime were 45.0%, 65.0% and 70.0%, respectively. The resistant rate to aminoglycosides antibiotics was 75.0%. The resistant rates to other antimicrobial agents were greater than 65.0%. The positive rate of genotype TEM, aac(3)-Ⅰ, aac(3)-Ⅱ aac(6”)-1 and ant(3”)-Ⅰ were 45.0%, 55.0%, 15.0%, 35.0%, and 60.0%, respectively. The genotypes of SHV, AmpC DHA and AmpC MIR were all tested negative.Conlusions The multiple-drug-resistance of Acinetobacter baumannii isolated from Tongde Hospital was a serious issue.There were high percentages of TEM and AMEs genotypes. Because of the imperfection frame of the primer,some familiar genes just like OXA,VER and PER were not experimented. We shall further discuss the drug resistance of the third/fourth generations cephalosporin.
