A high performance capillary electrophoresis method for determination of methotrexate in serum
- VernacularTitle:高效毛细管电泳法在血清氨甲蝶呤浓度检测中的应用
- Author:
Zuojun SHEN
;
Xiaodong HE
;
Ningling WANG
;
Zhimin ZHAI
;
Zimin SUN
- Publication Type:Journal Article
- Keywords:
Drug monitoring;
Methotrexate (MTX);
High performance capillary electrophoresis (HPCE)
- From:
Chinese Journal of Laboratory Medicine
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a rapid assay for the determination of methotrexate (MTX) in serum.Method The assay was based on the ultraviolet absorbance of methotrexate at 306 nm. The separation of the drug was done by high performance capillary electrophoresis (HPCE). The bare fused-silica capillary was 60 cm in total length, 50.5 cm in efficient length and 75?m in diameter. The voltage of 25 kV was applied. The running buffer was 75 mmol/L phosphate, pH7.4. The performance of methodology was evaluated.Result The complete separation of MTX was achieved within 10 min. The linearity of the assay was from 1.1 ?mol/L to 1100.0 ?mol/L. The minimal detection limit was 0.55 ?mol/L.The recovery of MTX was from 88.2% to 98.2%. Within-run precision was 4.2% and between-run precision was ~5.4%. Conclusion The result indicated that the method was an effective method for clinical and scientific research with advantages of rapidity, simplicity and accuracy.
