A enzymic method for the isolation and culture of rabbit bladder smooth muscle cells
- VernacularTitle:兔膀胱平滑肌细胞的分离、培养和鉴定
- Author:
Wen CHENG
;
Jianping GAO
;
Zhengyu ZHANG
;
Jun GONG
- Publication Type:Journal Article
- Keywords:
Rabbit;
Bladder;
Smooth muscle cell;
Culture
- From:
Journal of Medical Postgraduates
2004;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method of isolating,culturing and identifing in bladder smooth muscle cell of rabbit. Methods: Bladder smooth muscle cells were isolated from two adult male New Zealand White rabbits by collagenase digestion and cultured in DMEM medium supplemented with calf bovine serum. Morphology and expansion of the cells were observed. Cells were identified by smear of smear electron microscope and immunohistochemical methods(immunostaining with anti-?-actin antibodies). Results: The cells grew well and presented atypical morphological feature of smooth muscle cell with invert microscope(forming the "hills and valleys"). Ninety-nine percent of the cells were smooth muscle cells identified by smear of smear,electron microscope and immunohistochemical methods.HE stain and immunostaining. Conclusion:This bladder smooth muscle cell of rabbit cultural method is convenience, fruitful and reliable.
